METHOD, REAGENT AND KIT FOR THE DETECTION AND AMPLIFICATION OF NUCLEIC ACID SEQUENCES

摘要

Methods and reagents for the detection and exponential amplification of target nucleic acid molecules are disclosed. The method generally employs a Primer Oligonucleotide which hybridizes in concert with a Blocker Oligonucleotide on a strand of the target molecule, and an End-Run Oligonucleotide which can hybridize to the Blocker Oligonucleotide.