Detection of morphine using morphine dehydrogenase

摘要

PCT No. PCT/GB90/00727 Sec. 371 Date Nov. 12, 1991 Sec. 102(e) Date Nov. 12, 1991 PCT Filed May 11, 1990 PCT Pub. No. WO90/13634 PCT Pub. Date Nov. 15, 1990.Two enzymes, one an acetylmorphine carboxyesterase (AMCE), the other a morphine dehydrogenase (MDH), have been isolated from bacteria. The AMCE degrades heroin to morphine and the MDH oxidises morphine to morphinone, with the aid of a cofactor. These reactions are used in detection of heroin (using the two reactions coupled together) or morphine. The enzymes can be incorporated in sensors for this purpose.