摘要 |
<p>PURPOSE:To simply produce the subject peptide in high purity by subjecting a cysteine-containing peptide and a 3-nitro-2-pyridylsulfenyl group-protected cysteine-containing protected peptide to formation of a S-S bond, to formation of a peptide bond, and subsequently to removal of the protecting group. CONSTITUTION:A free SH group-having cysteine-containing protected peptide of formula I (X is amino-protecting group; A, B are various protected amino acid residues, protected peptide; (n), (m) are 0, 1) and a 3-nitro-2-pyridylsulfenyl group-protected cysteine-containing protected peptide are subjected to the formation of a S-S bond and subsequently to the formation of a peptide bond to produce a cysteine-containing protected peptide of formula III, which is subjected to a removal of the protecting group by a conventional method to provide the cysteine-containing peptide of formula IV (A', B', C', D' are the respective groups wherein the protecting groups are removed from A, B, C, D, respectively).</p> |