| 摘要 |
<p>Herein described is a new form of extraction collagen having the following characteristics: optical rotation, measured on 1 % autoclaved, in 0.5 M acetic acid, corresponding to a [α]D25°=-145° ± 5, toluidine blue metachromasia = negative, it reconstitutes by neutralization even from a solution in 0.1N HCl left at 37 °C during 60', it hydrolyses completely within 40 minutes by the action of pepsine, it is stable under the proteolytic action of pancreatin during over 24 hours. This form of collagen is obtained preceding its extraction from tissues by a treatment with sodium hydrate, and following extraction itself with a precipitation of collagen at pH 4.9 - 5.5 and a treatment with carbon dioxide. The thus obtained new form of collagen, among all known ones, is the closest to negative collagen and the most suitable for pharmaceutical and cosmetic use.</p> |
