ANTIBODIES SPECIFIC FOR A HAEMOSTATIC PROTEIN, THEIR USE FOR ISOLATING INTACT PROTEIN, HAEMOSTATIC COMPOSITIONS DEVOID OF PROTEOLYTIC CLEAVAGE PRODUCTS OF THE PROTEIN

摘要

Methods for the generation of Ca2+-independent antibodies against blood coagulation factors, employing an antibody selection strategy based on small peptides that are comprising target sequences for limited proteolysis. These antibodies, which substantially distinguish between intact and cleaved species of said haemostatic protein, provide novel tools for the isolation of intact haemostatic proteins. By virtue of the absence of cleavage products that usually are associated with sideeffects or reduced efficacy, these intact proteins may serve as improved agents in therapeutic compositions for the treatment of haemostatic disorders.