SPECIFIC ANTI-SALMONELLA MONOCLONAL REAGENTS, AND UNIQUE SEROLOGICAL APPROACH FOR THE DETECTION OF DIFFERENT COMMON SEROTYPES OF SALMONELLA AND THE LIKE

摘要

The detection and identification of Salmonella using murine hybridoma monoclonal antibodies is disclosed. A panel of anti-Vi and anti-O specific monoclonal antibodies has been successfully applied for the serotyping of Salmonella while anti-lipopolysaacharide core specific monoclonal antibodies have been applied for the detection of different common serotypes of Salmonella. Using a combination of the anti-O and anti-core specific monoclonal antibodies in a sandwich capture ELISA, it is possible to accomplish simultaneous detection and serogrouping of Salmonella. The immunoassay approach for the detection of Salmonella uses multiple time point assay, which measures a rise in the level of the targeted microorganism or its antigens, thereby allowing better differentiation of true positives from non-specific reactions in the immunoassay. This assay approach is therefore most suitable for immunoassay of infectious microorganisms as long as specific antisera or antibodies against the targeted microorganisms are available.