METHOD FOR INCREASING ACTIVITY OF ANTIBODY

摘要

PURPOSE:To obtain a highly active antibody free from sugar chain in variable portion and having remarkably increased antigen binding capacity and improved utility in the field of research, diagnosis and industry by cutting and removing sugar chain of a monoclonal antibody. CONSTITUTION:The highly active antibody free from sugar chain in variable portion and having remarkably increased antigen binding capacity and improved utility in the field of research, diagnosis and industry can be produced by treating a sugar chain existing in the antigen-recognizing site of a variable portion of a monoclonal antibody (e.g. human monoclonal antibody HB4C5) with a glycopeptidase in a 250mM sodium phosphate buffer solution containing 100mM 2-mercaptoethanol at 37 deg.C for 18hr, thereby cutting the sugar chain.