PROCEDIMENTO PER LA DETERMINAZIONE DI BIOMOLECOLE MEDIANTE UN SISTEMA DI AMPLIFICAZIONE ENZIMATICA.

摘要

Biomolecules such as antibodies, antigens or DNA, may be measured at particularly low concentrations by amperometry with the use of a marker enzyme such as alkaline phosphatase in conjunction with a new class of para- or ortho- hydroxyaryl phosphate substrates of general formula: in which Ar is phenyl or naphthyl, possibly substituted, which give rise to quinones by oxidation at the electrode. This system may be used in conjunction with a second enzymatic amplification system constituted by the measurement electrode itself and a glucose-glucose oxidase system which enables the limits of sensitivity of the biomolecular measurements to be lowered or the times for the determination to be reduced considerably.