Assay for 1,25 Dihydroxy Vitamin D.

摘要

An assay for 1,25-dihydroxy vitamin D is disclosed. The invention involves extracting blood serum using an organic solvent such as ethyl acetate, separating out potentially interfering other vitamin D metabolites using a silica column, and then adding pig receptor protein, radiolabeled 1,25-dihydroxy vitamin D, biotinylated antibody capable of binding to the receptor, and a facilitator protein such as BSA as part of an immunoprecipitation competitive binding assay. A kit for conducting this assay is also disclosed.