LABELING BY SIMULTANEOUS LIGATION AND RESTRICTION

摘要

LABELING BY SIMULTANEOUS LIGATION AND RESTRICTION Termini of restricted double-stranded DNA fragments are modified by ligating the fragments with terminal phosphate-free double-stranded oligonucleotides having a complementary terminus in the presence of a restriction enzyme and a ligase, where joining of the complementary ends results in loss of the restriction enzyme recognition sequence.