发明名称 |
ELECTRO-INSERTION OF PROTEINS INTO ANIMAL CELL MEMBRANES |
摘要 |
The present invention concerns a method of incorporating a protein into a cell membrane comprising exposing a cell to an electric field while the cell is suspended in an electro-insertion medium in the presence of a buffered solution (suspension) of the protein(s) to be inserted, the protein bearing a hydrophobic membrane spanning sequence and then contacting the resultant cell with a resealing medium.
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申请公布号 |
CA1327174(C) |
申请公布日期 |
1994.02.22 |
申请号 |
CA19890614874 |
申请日期 |
1989.09.29 |
申请人 |
TEXAS A & M UNIVERSITY SYSTEM;TEXAS A & M UNIVERSITY |
发明人 |
MOUNEIMNE, YOUSSEF;TOSI, PIERRE-FRANCOIS;NICOLAU, YVES-CLAUDE |
分类号 |
A61K9/50;C12N5/00;C12N5/06;C12N13/00;(IPC1-7):C12N5/00;C12N5/08;A61K35/12 |
主分类号 |
A61K9/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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