发明名称 |
Rat osteosarcoma cell line osr3tr1 |
摘要 |
The present invention relates to a novel cell line isolated from a rat osteosarcoma having the following characteristics: A) a non-expressed p53 protein; B) a normal RB-1 gene as compared to a non-tumorigenic Sprague-Dawley rat cell control; C) a normal c-fos gene as compared to a non-tumorigenic Sprague-Dawley rat cell control; D) a 50-fold amplified c-myc gene as compared to a non-tumorigenic Sprague-Dawley rat cell control; E) a monolayer MATRIGEL(Trademark) growth pattern; F) tumorigenic in nude mice; G) normal alkaline phosphatase activity; H) an ability to be serially propagated greater than fifty population doublings; I) an ability to produce a growth factor selected from the group consisting of: 1) a mesenchymal inhibitory growth factor; 2) a non-heparin binding mitogenic growth factor; 3) a first heparin binding mitogenic growth factor; 4) a second heparin binding mitogenic growth factor; 5) a third heparin binding mitogenic growth factor; and 6) a fourth heparin binding mitogenic growth factor. The present invention also relates to growth factors produced by the novel cell line. This invention also relates to a process for producing such growth factors.
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申请公布号 |
US5286645(A) |
申请公布日期 |
1994.02.15 |
申请号 |
US19920903434 |
申请日期 |
1992.06.24 |
申请人 |
THE PROCTER & GAMBLE COMPANY |
发明人 |
DOERSEN, CLAUS-JENS W.;ISFORT, ROBERT J. |
分类号 |
A61K38/00;C07K14/475;C12N5/09;(IPC1-7):C12N5/06;C12P21/02 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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