| 摘要 |
A method for a high-sensitivity quantitative analysis of bile acid in a bile acid-containing sample utilizes a reagent comprising: (1) a steroid dehydrogenase which is capable of effecting a reversible reaction producing oxobile acid using bile acid, as a substrate, and a nicotinamide adenine dinucleotide phosphate compound (hereinafter referred to as an NADP compound) or a nicotinamide adenine dinucleotide compound (hereinafter referred to as an NAD compound) as coenzyme; (2) a compound A1 selected from the group consisting of NADP compounds and NAD compounds, in an amount surplus relative to the amount of bile acid; (3) (i) compound B1 selected from the group consisting of a reduced NAD compound and a reduced NADP compound, compound B1 being a reduced NAD compound, when A1 is an NADP compound, and a reduced NADP compound, when A1 is an NAD compound, or (ii) a compound B2 which is an oxidized product of B1, or (iii) a mixture of B1 and B2, where the amount of B1 plus B2 is less than 1/100 of the molar amount of A1, and (iv) a second dehydrogenase which does not react with bile acid and compound A1, but does effect a reaction converting compound B2 into compound B1, and the substrate of the second dehydrogenase.
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