| 摘要 |
There is disclosed a process for isolating a recombinant polypeptide produced as a component of an insoluble fraction in a transformed host cell comprising entrapping the host cells within a porous matrix; disrupting the host cell membrane to enable diffusion of soluble host cell proteins from the host cell; washing the entrapped host cell to remove the soluble host cell proteins while leaving the insoluble fraction within the porous matrix; and contacting the insoluble fraction within the porous matrix with a chaotropic agent to isolate the recombinant polypeptide from the insoluble fraction. |
