RAPID DETECTION OF BIOPOLYMERS IN STAINED SPECIMENS

摘要

The present invention provides methods to detect the presence of a biopolymer in a previously stained specimen using novel (in situ) hybridization techniques. The method comprising assaying for the presence of cellular biopolymers, specifically RNA and DNA. The novel (in situ) hybridization procedure may be accomplished either in one or two (fixation followed by hybridization) steps. The methods of the present invention may be used to detect the presence of microorganisms, e.g., bacteria, viruses, fungi or cellular genes, e.g. oncogenes, tumor suppressor factors, or growth stimulatory factors in either previously stained cells or tissue.