Proteaseresistente Urokinase-Zusammensetzung, deren Herstellung und Verwendung.
摘要
Novel single-chain protease resistant urokinase derivatives are provided. In particular, derivatives are provided wherein the Lys135Lys136 and Arg156 to Lys158 sites are rendered less susceptible to proteolytic cleavage are provided by occluding the sites or by covalently modifying them. Preferred covalent modifications are amino acid sequence variants at the sites where proteolysis of urokinase occurs. These are optimally produced by synthesis of single-chain urokinase mutants in recombinant cell culture. The novel urokinase derivatives herein offer the advantage of avoiding the generation of substantial two-chain urokinase, either in vivo or during recombinant cell culture. However, the derivatives continue function to activate plasminogen in initiating blood clot lysis.
申请公布号
DE3689386(D1)
申请公布日期
1994.01.27
申请号
DE19863689386
申请日期
1986.04.21
申请人
GENENTECH, INC., SOUTH SAN FRANCISCO, CALIF., US
发明人
BLABER, MICHAEL, COSTA MESA CALIFORNIA 92627, US;HEYNEKER, HERBERT LOUIS, HILLSBOROUGH CALIFORNIA 94010, US;VEHAR, GORDON ALLEN, SAN CARLOS CALIFORNIA 94070, US