| 摘要 |
Essentially pure curculin B. A process for producing curculin B, comprising: culturing a transformed cell or microorganism containing a recombinant DNA containing a base sequence encoding curculin B, whereby curculin B is produced by said transformed cell or microorganism, and isolating curculin B from said transformed cell or microorganism. A DNA encoding mature or premature curculin B. A process for producing a DNA including a base sequence encoding curculin B, comprising: separating a fraction containing a curculin B mRNA from Curculigo latifolia, preparing a single-stranded DNA from said mRNA using reverse transcriptase, preparing a double-stranded DNA from said single-stranded DNA, inserting said double-stranded DNA into a vector, transforming a host with said vector to produce a cDNA library, and isolating a cDNA encoding curculin B from said library, using one or more synthesized DNA's containing a base sequence coding for a partial amino acid sequence elucidated from curculin A purified from Curculigo latifolia, as a probe. |
