| 摘要 |
PURPOSE:To produce riboflavin (vitamin B2) usefus as a drug, feed additive, and food colorant, by preliminarily culturing yeast belonging to Saccharomyces in a liquid, inoculating in a medium containing zinc ion, and continuing the cultivation. CONSTITUTION:The objective riboflavin is produced by inoculating a yeast strain belonging to Saccharomyces genus and capable of producing riboflavin [e.g. Saccharomyces cerevisiae (Candida robusta AHU 3405), etc.] in a liquid medium for preculture, culturing at 30 deg.C for 1-2 days under shaking, inoculating in a medium containing preferably 0.1-100mg, of zinc ion per 1 liter, and culturing preferably at 6-8pH and 20-37 deg.C under aerobic condition. |
