Temporary inactivation of serine hydrolases using nitrophenyl phenacyl phosphonates

摘要

Phenacyl phosphonate adducts of serine hydrolases temporarily inactivate the hydrolases. The hydrolases are reactivated by intramolecularly catalyzed dephosphonylation of the adduct over a period of time. Half-lives for dephosphonylation in the range of about 30 to 500 minutes have been observed at physiological pH's.