| 摘要 |
PURPOSE:To provide the novel enzyme useful for hydrolyzing cephalosporin C to produce aminocephalosporanic acid, and having a high enzymatic activity and a high processing effect. CONSTITUTION:An enzyme of the formula [A<1-268> is an amino acid sequence ranging from the Thr<1> of natural cephalosporin C acylase (CCAase) to its Cly<268>; A<272-304> is an amino acid sequence ranging from the Cln<272> of CCAase to its Tyr; A<306-773> is an amino acid sequence ranging from the Val<306> of CCAase to its Ala<773>; X1-X3 are amino acids, but when X1 and X2 are Met and Ala, respectively, X3 is the groups excluding Cys]. The enzyme is obtained by a recombination DNA method comprising transforming a host cell with an expression vector recombined with a DNA coding the amino acid sequence, culturing the host cell, and subsequently separating the produced enzyme from the cultured product, or by a polypeptide synthetic method, etc. |
