| 摘要 |
PURPOSE: To improve the effect of the decomposition of one-chain DNA molecules by providing the specified relation between the values of the potential gradient and the period of alternating front electric field and reverse electric field. CONSTITUTION: In the method for the electrophoresis separation of the mixture of one-chain DNA molecules of the base, whose length is 200 or more along the moving direction selected as a reserve in an electrophoresis separation supporter, the front electric field and the reverse electric field, which is rotated by 180 deg., are alternated so as to cross the electric field of the supporter. The potential of the alternating reverse pulse is higher than the potential of the front pulse. The continuing period of the front pulse is longer than the continuing period of the reverse pulse. The relationship of them is expressed by the expression. In the expression, Ef and tf are the potential gradient and the continuing period of the front purse, and Er and tr are the potential gradient and the continuing period of the reverse pulse. Furthermore, Ef and Er are larger than about 10V/cm or equal, and tf and tr are made to be larger than at least about 0.01 second or equal. The optimum potential gradient or potential gradient ratio for the specified se paration are changed in dependence on the size of the one-chain DNA to be separated. |
