发明名称 |
PURIFIED SACCHAROSE SYNTHASE, PROCESS FOR ITS PRODUCTION AND ITS USE |
摘要 |
Nucleotide sugars, especially UDP, ADP, CDP or TDP saccharoses can be enzymatically obtained by the reaction of nucleoside diphosphates with di or trisaccharides with a saccharose synthase in which the virtual absence of nucleoside phosphatases (0.1 % or less) can be ensured by special purification methods and sensitive detection. The purification of the raw extract, obtained preferably from rice grains, comprises especially the application of the ultra-filtered extract containing 50 mM KCl with a pH 8 on a sepharose Q column and a gradient elution out of the column at a pH 8 with 50 to 500 mM KCl. |
申请公布号 |
WO9401540(A1) |
申请公布日期 |
1994.01.20 |
申请号 |
WO1993DE00562 |
申请日期 |
1993.06.26 |
申请人 |
FORSCHUNGSZENTRUM JUELICH GMBH |
发明人 |
ELLING, LOTHAR;KULA, MARIA-REGINA |
分类号 |
C07H19/10;C07H19/20;C12N9/10;C12P19/30;C12P19/38 |
主分类号 |
C07H19/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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