VACCINE COMPRISING MIXED preS1+preS2+S AND CORE PARTICLE

摘要

To produce hepatitis B virus (HBV) surface and core proteins as mixed particles, DNA encoding HBV proteins is expressed in a single recombinant yeast. To form particles with substantially reduced carbohydrate, DNA encoding HBV proteins is expressed in a single recombinant yeast which is glycosylation deficient. These HBV proteins display, on the same mixed particle, antigenic sites genetically encoded by the envelope domains (including preS and S) and the core antigen. The particles also contain substantially reduced nucleic acid content. These particles are useful as a vaccine for active and passive treatment or prevention of HBV disease and/or infection and serologically related agents including surface protein antigenic variants (especially in populations hypo- or non-responsive to other HBV vaccines), and also as reagents for use in diagnostic tests.