A PROCESS FOR RECOVERING A RECOMBINANT PROTEIN, IN BIOLOGICALLY ACTIVE FORM, FROM A SOLUTION CONTAINING INACTIVE PROTEIN

摘要

A process for obtaining a recombinant protein in its natural and bioactive form from a solution containing inactive protein, present in the form of either an insoluble inclusion body or oxidized, misfolded and aggregated waste protein comprises diluting the solution of inactive protein with a first alkaline buffer solution, adding SDS and L-cysteine to simultaneously solubilize and reduce the protein, decreasing the concentration of the SDS and L-cysteine by about 70-90 % by diafiltration against a second alkaline buffer solution, and oxidizing the denatured and reduced protein to form disulfide bonds which correspond to those present in the naturally occurring bioactive protein.