| 发明名称 |
ALKALINE PHOSPHATASE ENZYMES HAVING IMPROVED SPECIFIC ACTIVITY FOR USE IN INDICATOR REAGENTS |
| 摘要 |
The present invention involves the construction of several mutations in the Escherichia coli alkaline phosphatase gene, that result in the production of an enzyme having an improved specific activity. The resultant mutant enzymes have up to 1.5- to 36-fold higher specific activity, while retaining a temperature stability that is significantly higher than that of mammalian alkaline phosphatase enzymes. The utility of mutant E. coli alkaline phosphatase as an enzyme label in binding assays is also demonstrated. |
| 申请公布号 |
WO9401531(A1) |
申请公布日期 |
1994.01.20 |
| 申请号 |
WO1993US06264 |
申请日期 |
1993.07.01 |
| 申请人 |
ABBOTT LABORATORIES |
发明人 |
MANDECKI, WLODZIMIERZ;SHALLCROSS, MARY, ANN;TOMAZIC-ALLEN, SUSAN, J. |
| 分类号 |
G01N33/535;C12N1/15;C12N1/21;C12N5/10;C12N9/16;C12N15/09;C12Q1/42;C12R1/19;G01N33/543;G01N33/58;(IPC1-7):C12N1/15;C12Q1/34;C12N15/63;C12N15/55;C12N5/16;C12N1/19;C07H21/04 |
主分类号 |
G01N33/535 |
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