发明名称 |
Method for enhancing recovery of recombinant proteins |
摘要 |
The present invention relates to a method for enhancing the amount of recombinant protein recovered after fermentation of bacteria containing vectors encoding the recombinant protein. The method is not limited by a required induction of the cloned gene by addition of exogenous inducers. Using the methods of the invention, bacterial recombinant protein production can exceed a level of 20 % of the total soluble protein expressed. The methods of the invention are demonstrated on bacterial cytochrome P450 and on bacterial electron transfer proteins typically difficult to express to commercially feasible levels. |
申请公布号 |
GB2256870(B) |
申请公布日期 |
1994.01.19 |
申请号 |
GB19920018037 |
申请日期 |
1992.08.25 |
申请人 |
* BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM |
发明人 |
J A * PETERSON;S S * BODDUPALLI |
分类号 |
C12N1/21;C12N9/02;C12N15/53;C12N15/67;C12P21/02 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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