发明名称 |
Exopeptidase catalyzed site-specific bonding of supports, labels and bioactive agents to proteins |
摘要 |
The invention provides a means for attaching a label, support or bioactive agent to the protein at a site that is remote from the active site of the protein. More specifically the invention is directed to a method for the attachment of an amino acid, amino acid derivative, amine and alcohol nucleophile to the carboxyl terminus of a protein. In one embodiment, an exopeptidase enzyme is utilized to attach a labeled nucleophile to a protein such as an antibody. In other embodiments, the invention is directed to a method for the attachment of a protein to an immobilization support and to a method for the attachment of a bioactive agent to a protein.
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申请公布号 |
US5279954(A) |
申请公布日期 |
1994.01.18 |
申请号 |
US19890375138 |
申请日期 |
1989.06.30 |
申请人 |
BOARD OF REGENTS OF THE UNIVERSITY OF NEBRASKA AND BIONEBRASKA |
发明人 |
WAGNER, FRED W.;COOLIDGE, THOMAS R.;WYLIE, DWANE E.;SCHUSTER, SHELDON M.;LEWIS, WILLIAM;STOUT, JAY |
分类号 |
C07K17/06;C07K1/107;C07K14/575;C07K16/00;C07K16/40;C07K17/00;C07K19/00;C12N11/00;C12N11/02;C12N11/06;G01N33/532;G01N33/543;G01N33/58;(IPC1-7):C12N11/14;G01N33/551;G01N33/544 |
主分类号 |
C07K17/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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