| 摘要 |
<p>PURPOSE:To collect a highly productive strain useful for medicines, etc., by plant tissue culture rapidly, simply and efficiently by making shoots into calluses, dividing, culturing a strain under a condition of producing secondary metabolites and selecting a strain having a high content of the secondary metabolites. CONSTITUTION:Shoots such as one derived from a plant of the genus Digitalis are made into calluses once, returned to shoots again and the shoots are divided. Each strain is cultured under a condition of producing secondary metabolites such as digitoxin, digoxin, lanotoside C, purpurea glycoside A, digitoxigenin, digoxigenin or deacetyl lanatoside C and a strain having high content of the secondary metabolite is selected from the culture mixture to collect a highly productive strain by plant tissue culture.</p> |
