| 摘要 |
This invention pertains to a set of polypeptide antigens having amino acid sequences derived from amino acid sequences of a population of variants of a protein, or a portion thereof, and to methods of producing the set of polypeptide antigens. In general, the method comprises (i) selecting a protein, or a portion thereof, which exhibits a population of N variants, represented by the formula A1A2A3 ... An-2An-1An, where An is an amino acid occurring at amino acid position n of the protein, or portion thereof; (ii) determining the number of times Onaa each type of amino acid occurs at each amino acid position n in the N variants; (iii) calculating the frequency of occurrence (Onaa/N)n of each type of amino acid at each amino acid position n in the N variants; and (iv) generating a set of polypeptide antigens having amino acid sequences represented substantially by the formula A'1A'2A'3 ... A'n-2A'n-1A'n, where A'n is defined as an amino acid type which occurs at greater than a selected frequency at the corresponding amino acid position in the N variants. |
