| 摘要 |
<p>Carrier-free [<3><2>P] inorganic phosphate is incorporated into the gamma -phosphate of ATP and other nucleoside triphosphates by a series of enzymatic reactions in the presence of dihydroxyacetone phosphate, NAD<+>, and their corresponding nucleoside diphosphates. NAD<+> is preferably regenerated by malate dehydrogenase and oxaloacetate. The resulting [ gamma -<3><2>P] nucleotides are useful as reagents for analytical determinations. [ gamma -<3><2>P] ATP is also useful for preparing [ alpha -<3><2>P]-labeled nucleotides having high specific activity.</p> |
