摘要 |
The mixtures used for the detection reaction are obtained as a simple addition of oligonucleotides, contain homologous sequences selected amongst related genomic sequences and may comprise non homologous sequences and/or changes with respect to known sequences. The mixtures used for the identification reaction, consecutive to the detection or not, are obtained by addition of oligonucleotides, terminate at their extremity 3' by a sequence specific to the sequence to be typified, are configured so that the specific amplified fragments are different between each other as to the size or as to any other physical or chemical marking and may comprise changes with respect to known sequences. The new processes and mixtures are specially used for the detection and typing of related infectious agents, specially related viruses. |