发明名称 |
RECOMBINANT PRODUCTION OF SAPORIN-CONTAINING PROTEINS |
摘要 |
2138038 9325688 PCTABScor01 Methods for the recombinant production of saporin-containing proteins, including cell surface binding protein-saporin fusion proteins, are provided. The resulting fusion proteins are cytotoxic to targeted cells. In preferred embodiments, methods are provided for the production of basic fibroblast factor (bFGF)-saporin fusion proteins by culturing Escherichia coli that has been transformed with a vector containing DNA encoding bFGF linked via a spacer peptide to the amino terminus of a cytotoxic portion of a saporin polypeptide to obtain expression of the DNA, and isolating the resulting FGF-saporin fusion protein. FGF-saporing fusion proteins and saporin proteins containing from about 5 to 12 amino acid N-terminal extensions are also provided.
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申请公布号 |
CA2138038(A1) |
申请公布日期 |
1993.12.23 |
申请号 |
CA19932138038 |
申请日期 |
1993.06.14 |
申请人 |
WHITTIER INST DIABETES & ENDOC;PRIZM PHARMA INC |
发明人 |
LAPPI DOUGLAS A;BARTHELEMY ISABEL;BAIRD J ANDREW;SOSNOWSKI BARBARA A |
分类号 |
C12N15/09;A61K39/00;C07K1/20;C07K1/22;C07K14/415;C07K14/50;C07K14/575;C07K16/00;C07K19/00;C12N1/21;C12N15/29;C12P21/02;C12P21/04;C12R1/19;(IPC1-7):C12N15/62;C12N9/00;C12N15/13;C12N15/16;C12N15/52;C12N15/70 |
主分类号 |
C12N15/09 |
代理机构 |
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地址 |
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