| 摘要 |
A gene, sod2, which is capable of conferring sodium and lithium tolerance in Schizosaccharomyces pombe has been identified and characterized. A plasmid, psod2, consisting of about 5.8 kb of S. pombe wild type genomic DNA inserted in a plasmid vector pFL20 has been isolated and used to transfer sodium and lithium tolerance to wild type S. pombe. A mutant strain of S. pombe, sod2-1, having sodium and lithium tolerance has been selected and shown to have an amplified locus. The amplified locus corresponds to the genomic insert of psod2. The gene or homologues thereof may be inserted into higher life forms, such as plants.
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