| 摘要 |
PURPOSE:To make it possible to separate and refine material related organism such as nucleic acid by using silica-based filler containing flourine, which is treated with guarternary ammonium salt expressed by the specified formula. CONSTITUTION:Silica-based filler containing flourine is treated with quarternary ammonium salt expressed by the formula I and used. (In the formula, R1-R4 indicate the alkyl groups, whose carbon number is 1-30, and can have fluoroalkyl groups. X indicates the anion of inorganic acid, and (a) indicates the integer of 1-3.) Or the filler is treated with quarternary ammonium salt expressed by the formula II. (In the formula, Y1-Y4 are hydrogen atoms or fluorine atoms, and (m), (o), (r) and (s) are 0-12 and all are not 0 at the same time. The letters (n), (p), (q) and (t) indicate the integers of 0-12. X<b-> indicates the aninon of inorganic acid, and (b) indicates the integer of 1-3.) The silica-based filler containing fluorine is dispersed into solvent such as chloroform. Then the quarternary ammonium is added. Thereafter, an evaporator is used, and the chloroform is removed. Thus, the intended filler can be manufactured. |
