Quantitation of viral rna by competitive polymerase chain reaction

摘要

The claimed invention relates to a method for quantitating virus RNA in a biological sample using a competitive PCR reaction. The method employs an internal standard RNA which is included in the PCR reaction in order to control for variability in the amount of PCR product from reaction to reaction. The method is particularly useful for monitoring plasma levels of virus RNA. The invention also relates to a cloned nucleic acid sequence useful for producing the standard RNA used in the method claimed.