| 摘要 |
PCT No. PCT/US93/04847 Sec. 371 Date Dec. 12, 1994 Sec. 102(e) Date Dec. 12, 1994 PCT Filed May 21, 1993 PCT Pub. No. WO93/23534 PCT Pub. Date Nov. 25, 1993A process for insertional mutagenesis and genome manipulations of yeast cells. In the first step of this process, viable yeast cells are combined with a restriction enzyme cleaved deoxyribonucleic acid fragment which lacks substantial sequence identity and shows no specific hybridization signals with the DNA of the viable yeast cells. The viable yeast cells are then transformed so that the cleaved deoxyribonucleic acid fragment is incorporated into the yeast cells by nonhomologous recombination; and the transformed yeast cells are then incubated in the presence of a growth medium. |
