| 摘要 |
The present invention relates to a method for identification and detection of pectinolytic bacteria of the genus Erwinia of predetermined species, subspecies or pathovar, and more particularly of the species Erwinia chrysanthemi and of the species Erwinia carotovora, in the ground or in the water, or in a host susceptible of being the carrier of such bateria, particularly in plants and seeds, such process comprising assaying for fragments of DNA or RNA, which are specific of the concerned species, coding a pectate-lyase (PL), through a molecular hybridization with at least one specific probe, such hybridization being preceded by an amplification of the number of copies of the above-mentioned DNA fragments by means of specific primers. The invention also relates to probes and primers as well as to kits containing them for implementing such method.
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