| 摘要 |
<p>A pipette tip (30) for use with a pipetter and a method for dispensing a precise volume of a fluid (54). The pipette tip (30) has a hollow body (32) which defines an interior volume (34), and a channel (42) therein having a calibrated volume. Channel (42) extends from an intake opening (44) to an overflow opening (46) positioned inside the interior volume (34) of the tip above a reservoir portion (50) inside the tip. Fluid (54) drawn through the intake opening (44) and into the channel (42) in excess of the calibrated channel volume is gravity-shed or sloughed into an overflow reservoir (50). Thus, a calibrated volume of fluid (54) remains in the channel (42) and can be dispensed accurately from the channel (42). Additionally, the reservoir portion (50) captures and contains an overflow fluid (56) in a stable condition, permitting the reservoir (50) to be used to react various substances with the overflow fluid (56) inside the pipette tip (30). The internal reservoir (50) also may be divided by a partition to provide a plurality of reservoirs or sub-volumes inside the pipette tip (30).</p> |
