| 摘要 |
PURPOSE:To obtain a new nucleotide having a basic sequence which is a part of main outer coat protein genes of Chlamydia, useful as a primer used in detection of microorganisms of the genus Chlamydia utilizing an amplifying method by a polymerase chain reaction. CONSTITUTION:A nucleotide having the sequence of the formula or a derivative obtained by carrying out at least one change comprising a change by removing 1 or 2 bases from the end on 5' side in the sequence of the formula or a change removing 1 or 2 bases from the end on 3' or change adding -T or -TA to the end and change replacing 1 or 2 bases other than both ends with other base selected from A, C, G and T. The nucleotide having sequence of the formula is obtained e.g. by synthesizing using a DNA synthesizer and then purifying with a high-performance liquid chromatography. |
