发明名称 |
INOSINIC ACID DEHYDROGENASE ASSAY |
摘要 |
A cyto(histo)chemical technique for determining inosine 5'-monophosphate dehydrogenase (IMPD) presence and concentration within one or more morphologically classified or classifiable cells taken from an animal or human subject. Cell monolayers are prepared, fixed and stained according to a specified procedure in which inosine 5'-monophosphate (as enzyme substrate), nicotinamide adenine dinucleotide (NAD), phenazine methosulfate (PMS) and nitro blue tetrazolium (NBT) as a diazonium salt together produce a granular, dark blue deposit of formazan generally proportional to the IMPD activity in the cell. Cells are evaluated either visually or by image analysis, with a computerized image analyzer or other imaging system, according to accepted biostatistical methods and standards. |
申请公布号 |
WO9323558(A1) |
申请公布日期 |
1993.11.25 |
申请号 |
WO1993US04641 |
申请日期 |
1993.05.14 |
申请人 |
THE MEDICAL COLLEGE OF PENNSYLVANIA |
发明人 |
MARKOVIC, OLIVERA, T.;MARKOVIC, NENAD, S.;ROBERTS, JAY;PUGLIA, CHARLES, D.;MARKOVIC, SVETOMIR, N. |
分类号 |
C12Q1/32;G01N33/50;G01N33/574;(IPC1-7):C12Q1/02 |
主分类号 |
C12Q1/32 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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