| 摘要 |
<p>This invention provides a purified protein having an estimated molecular weight of about 55 to 65 kD under reducing conditions, or an active fragment thereof, wherein the protein binds renin and wherein the renin-binding activity can be deactivated in vitro in inside-out membrane vesicles incubated in Krebs-Ringer buffer with high potassium, by phospholipase A2, lysolecithin, melittin, trypsin, calcium, barium and potassium chloride and wherein the renin-binding activity can be activated by magnesium and calmodulin. Also provided is a method of predicting an increased probability of a pathology associated with low renin syndrome in a subject comprising quantitating the amount of renin in a specimen from the subject, the presence of renin below about 1 ng/ml plasma per hour of incubation indicating the presence of a disease associated with the syndrome.</p> |
