IMMOBILIZED ENZYME FILM, PROTEIN IMMOBILIZED FILM AND PROCESS FOR FORMING THE SAME

摘要

An immobilized enzyme is formed using an enzyme solution prepared by adding 1 to 3 parts by weight of a 50 to 100 wt % water-soluble crosslinking agent having at least two epoxy groups in the molecule and 1 to 3 parts by weight of a 1 to 2 wt % glutaraldehyde to 1 to 3 parts by weight of a 10 to 50 wt % aqueous protein solution containing an enzyme, and a process for forming the same on an ion-sensitive field effect transistor. The thus-formed immobilized enzyme film (12) can be used in an enzyme electrode and is of a uniform thickness and stable by virtue of its increased hydrophilicity and higher elasticity, whereby deactivation of the enzyme to be caused by the strinkage of the film can be prevented.