| 摘要 |
PURPOSE:To obtain in an industrially advantageous way the subject compound effective for learning function improvement, dementia curing, etc., useful for health foods or medicines by culture of Emiliania algae and by collecting docosahexaenoic acid-contg. lipids from the cultured algae. CONSTITUTION:An inorganic liquid medium is inoculated with Emiliania algae (e.g. Emiliania huxley) and the system is put into a flat glass vessel with its flat side provided with fluorescent lamp(s), and the algae is cultured for 3 days under light irradiation intermittently with bright period of 16 hr and dark period of 8 hr so as to be >=5 klux in the surface illumination for the vessel followed by bringing the surface illumination to <5 klux, and the culture is made for additional three days with the conditions other than the illumination fixed. Then, the cultured algae is collected through centrifugalization, washed with water, and lyophilized. The algae thus lyophilized is heated in a 5% HCl methanol solution to methylate the fatty acid in the algae followed by extraction, and the resulting extract is hydrolyzed and separated, thus obtaining the objective docosahexaenoic acid. |
