| 摘要 |
<p>PURPOSE:To provide the monoclonal antibody recognizing human urine protease- inhibiting protein (UTI) which relates to cancer diseases and which is contained in the urine, and further to provide the method for determining the UTI with the antibody. CONSTITUTION:A mouse is subjected to an immunological treatment using an antigen containing the UTI. The splenic cell of the immunized mouse is fused with a myeloma cell originated from a mouse, and an UTI-resistant monoclonal antibody-producing hybridoma cell is screened from the fused cells. The hybridoma cell is administered into the abdominal cavity of a mouse, and the abdominal dropsy of the mouse is collected. The collected fraction is purified into the monoclonal antibody recognizing the UTI. The monoclonal antibody is reacted with a specimen containing urinary proteins, and an antibody bonded to a label molecule for color generation is bonded to the above- mentioned monoclonal antibody to develop the color of the label molecule for the colorimetry of the UTI. The method is clinically useful for the diagnosis of the disease state of a cancer patient and as an indicator for the process of the treatments of the patient.</p> |
