Terminal modifications of tumor necrosis factor

摘要

PCT No. PCT/JP89/00966 Sec. 371 Date Mar. 22, 1991 Sec. 102(e) Date Mar. 22, 1991 PCT Filed Sep. 22, 1989 PCT Pub. No. WO90/03395 PCT Pub. Date Apr. 5, 1990.This invention relates to a novel physiologically active polypeptide containing modified amino acids on both terminals at the amino-terminus and the carboxy-terminus in the amino acid sequence of the human tumor neclosis factor (human TNF), a recombinant plasmid containing a DNA region encoding the polypeptide, a recombinant microorganism cell transformed with the recombinant plasmid, a method of producing the polypeptide by cultivating the microorganism cell, a pharmaceutical composition containing the polypeptide and a method of purifying and recovering the polypeptide. The polypeptide in this invention has excellent antitumor activity compared to the human TNF and can be utilized as an active ingredient of a pharmaceutical composition having antitumor activity.