Capillary electrophoresis buffer

摘要

Disclosed herein is a dynamic coating buffer useful in the analysis of samples by open-tube capillary electrtophoretic methods using untreated fused silica capillary columns. The dynamic coating buffer comprises at least one agent having at least two dissociation constants, and high ionic strength characteristics, said agent having a molarity range of from about 0.2M and about 1.0M and a pH range of from between about 3.0 and 11.0. A particularly preferred dynamic coating buffer comprises 0.5M sodium phosphate.