| 摘要 |
Disclosed is a method that relates to the measurement of determinants related to the in-vivo induction of spermidine/ spermine Nl-acetyltransferase (SSAT), subsequent to treatment with polyamine analogs that induce SSAT such as bis-ethyl spermine analogs, in tumor types responsive to this class of analogs. The method comprises the measurement of one or more SSAT-specific determinants that include SSAT enzyme activity, SSAT enzyme protein, and SSAT m-RNA transcripts. Alternatively, other determinants related to the SSAT induction may be measured. Such determinants include SSAT co-factor acetylCoenzyme A, and SSAT products Nl-acetylspermidineand Nl-acetylspermine. Measurements of these determinants may be useful as prognostic indicia and tumor response markers to evaluate the clinical effectiveness of anticancer agents comprising polyamine analogs, such as bis-ethyl spermine analogs, that induce SSAT activity.
|
