| 摘要 |
<p>A quantitative viral assay comprises capturing virus particles, releasing viral RNA or DNA and, where necessary, reverse transcribing viral RNA to complementary DNA (cDNA), amplifying the viral DNA or cDNA by a nested multi-stage PCR reaction, capturing the PCR product using an immobilised binding partner specific to a binding agent introduced in the PCR product and quantifying the captured PCR product. The assay is particularly useful in the determination of viral load and in the determination of the relative proportions of different viral forms, for example as produced by a point mutation. The assay may be used to follow the development of drug resistance.</p> |
