发明名称 |
The complete gene (cefG) encoding the acetyl-CoA: deacetylcephalosporin C acetyltransferase of Cephalosporium acremonium, its isolation and use. |
摘要 |
The gene (cefG) encoding the acetyl-CoA: deacetylcephalosporin C acetyltransferase (DAC-ATF) of Cephalosporium acremonium (syn. Acremonium chrysogenum) C10 has been isolated. It contains two introns and encodes a protein of 444 amino acids with a molecular weight of 49269 that correlates well with the molecular weight deduced by gel filtration (Mol. weight 52 000 +/- 1 000). The cefG gene is linked to the cefEF gene (encoding the bifunctional deacetoxycephalosporin C synthase/hydroxylase) but it is expressed in the opposite orientation to the cefEF gene. The isolated cefG complements the deficiency of deacetylcephalosporin acetyltransferase e.g. in the non-producer mutant C. acremonium ATCC 20371 and restores cephalosporin biosynthesis in this strain. The cefG is therefore useful to improve cephalosporin C production. <IMAGE> |
申请公布号 |
EP0566897(A2) |
申请公布日期 |
1993.10.27 |
申请号 |
EP19930105016 |
申请日期 |
1993.03.26 |
申请人 |
HOECHST AKTIENGESELLSCHAFT |
发明人 |
MARTIN MARTIN, JUAN FRANCISCO, PROF. DR.;GUTIERREZ MARTIN, SANTIAGO;VELASCO ALVAREZ, JAVIER;FERNANDEZ PERRINO, FRANCISCO JOSE |
分类号 |
C12N9/10;C12N15/54;C12N15/80;C12P35/06 |
主分类号 |
C12N9/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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