| 摘要 |
<p>PURPOSE:To obtain a new substance having IFN-gamma inducing activity, recognized to have antitumor effects. CONSTITUTION:An active substance having the following properties. Molecular weight: 70 kilodalton. Reactivity with monoclonal antibody TS-2 and inducing ability of IFN-gamma are not deactivated by heat treatment at 56 deg.C for 30 minutes, treatment with 0.2mg/ml pronase at 37 deg.C for 1 hours, treatment with 0.5IV/ml neueraminidase at 37 deg.C for 2 hours and treatment with 0.15M-NaCl-ammonia (pH 11) and deactivated by treatment with 50mM periodic acid at 4 deg.C for 2 hours and treatment with 0.2M glycine-HCl (pH2.5). The active substance is obtained by extracting hemlytic streptococcal OK-432 with butanol by Morrison method to give a glycolipid product, separating a substance from the glycolipid product by using a monoclonal antibody TS-2 as a ligand by affinity chromatography, then separating into 25 fractions by Sephacryl-S-300 column to give a substance corresponding to the fraction 8 and collecting the active substance from the substance.</p> |
